In Vitro Evaluation Of Antacid Experiment Lab

 In Vitro Evaluation Of Antacid

The Introduction

     Antacids are chemical substances which on ingestion act by ,  neutralization of  gastric juice to form a neutral salt, it provides symptomatic  relief

The Procedure

  To initiate the physiological conditions in the stomach, artificial gastric juice is 
used 

 (Artificial Gastric Juice( A.G.J

     Sodium chloride        2gm
   HCl                            7 ml
D.W.          to             1000ml

Place 150 ml of the (AGF) in a 250 ml beaker

Arrange the pH meter so that the electrode are in the solution

 Fill a 50 ml burrette with (AGF) and position it so that solution may be added to the beaker as desired

Record the initial pH of the juice

 Add the antacid and stir the solution (3 gm of citrogram and 15 ml of Maalox)

Record the pH at 2 min. intervals

 To stimulate stomach condition, add 2ml of fresh juice from the burette each 2 min. and at the end of each 10 min., withdraw 10 ml from the beaker using a pipette and discord

 Continue recording until the pH gas reached to its maximum and then dropped

 significantly, record for 50 min

Draw a curve by plotting pH versus time for both antacids on the same plot and 

compare

The Antacids Used In Our Experiment

Fwar Lemon contain 6 sachets

 

 Each sachet - 5 gm- contain

sodium bicarbonate         2.8 gm

Tartaric acid                    1.33 gm

Citric acid                       0.82 gm

Flavour            to            5.00 gm

Dosage

For adult: one sachet to be added to half a glass of water & taken while effervesing after meals

For children: half the adult dose

Made in Egypt

Pharco Pharmaceutical  - Alexandria

فوار ليمون: يحتوي ٦ أكياس

ملح فوار يستعمل لعلاج الأنتفاخ وعسر الهضم والحموضة

خال من السكريات

طريقة الأستعمال: تضاف محتويات الكيس الى نصف كوب ماء وتؤخذ اثناء الفوران

Eno Powder (Fruit Salt) 150 gm

Fast Refreshing Relief From Stomach Upset

Each 5 gm of Eno powder contains

Sodium bicarbonate       B.P.          2.32 gm

Citric acid                        B.P.         2.18 gm

Anhydrous sodium carbonate        0.5  gm

Dose

For adults and children  (aged 12 years and over)

one teaspoon (5 ml) in water every 2-3 hours, if necessary

Notes

Minimum dosing interval: 2 hours

Max. daily dose (MDD): 6 * 5 ml

Max. duration of antacid use at MDD: 14 days

 Eno Powder contain sodium (0.85 gm per each 5 gm) persons on a sodium restricted diet should not take multiple doses of this product unless directed by a doctor