In Vitro Evaluation Of Antacid Experiment Lab

 In Vitro Evaluation Of Antacid

The Introduction

     Antacids are chemical substances which on ingestion act by ,  neutralization of  gastric juice to form a neutral salt, it provides symptomatic  relief

The Procedure

  To initiate the physiological conditions in the stomach, artificial gastric juice is 
used 

 (Artificial Gastric Juice( A.G.J

     Sodium chloride        2gm
   HCl                            7 ml
D.W.          to             1000ml

Place 150 ml of the (AGF) in a 250 ml beaker

Arrange the pH meter so that the electrode are in the solution

 Fill a 50 ml burrette with (AGF) and position it so that solution may be added to the beaker as desired

Record the initial pH of the juice

 Add the antacid and stir the solution (3 gm of citrogram and 15 ml of Maalox)

Record the pH at 2 min. intervals

 To stimulate stomach condition, add 2ml of fresh juice from the burette each 2 min. and at the end of each 10 min., withdraw 10 ml from the beaker using a pipette and discord

 Continue recording until the pH gas reached to its maximum and then dropped

 significantly, record for 50 min

Draw a curve by plotting pH versus time for both antacids on the same plot and 

compare

The Antacids Used In Our Experiment

Fwar Lemon contain 6 sachets

 

 Each sachet - 5 gm- contain

sodium bicarbonate         2.8 gm

Tartaric acid                    1.33 gm

Citric acid                       0.82 gm

Flavour            to            5.00 gm

Dosage

For adult: one sachet to be added to half a glass of water & taken while effervesing after meals

For children: half the adult dose

Made in Egypt

Pharco Pharmaceutical  - Alexandria

فوار ليمون: يحتوي ٦ أكياس

ملح فوار يستعمل لعلاج الأنتفاخ وعسر الهضم والحموضة

خال من السكريات

طريقة الأستعمال: تضاف محتويات الكيس الى نصف كوب ماء وتؤخذ اثناء الفوران

Eno Powder (Fruit Salt) 150 gm

Fast Refreshing Relief From Stomach Upset

Each 5 gm of Eno powder contains

Sodium bicarbonate       B.P.          2.32 gm

Citric acid                        B.P.         2.18 gm

Anhydrous sodium carbonate        0.5  gm

Dose

For adults and children  (aged 12 years and over)

one teaspoon (5 ml) in water every 2-3 hours, if necessary

Notes

Minimum dosing interval: 2 hours

Max. daily dose (MDD): 6 * 5 ml

Max. duration of antacid use at MDD: 14 days

 Eno Powder contain sodium (0.85 gm per each 5 gm) persons on a sodium restricted diet should not take multiple doses of this product unless directed by a doctor

Preperation of a caliberation curve of salicylic acid with the Application of statistics Lab

Biopharmaceutics Lab.1

Preperation of a caliberation curve of salicylic acid with the Application of statistics

Caliberation curve: is a curve prepared from a series of standard solution and used as a reference curve to obtain the concentration of unknown sample of the same drug.

The curve should be fitted either:

1- By eye fitting  or

2- By least square fitting (most reliable method)

The curve is based on the equation which minimize the summation of the square deviation of the observed value from the line [Σ(y- ȳ)²], where y= observed value & ȳ = calculated value.                                                                                         

Note:

The X- axis is the Independent variable (conc.)

The Y- axis is the dependent variable (absorbance)

Linear Regression Line

ȳ= c + bx

b= (Σx.y) – [[Σx.(Σy/n)]/(Σx²)-[[( Σx)²/n]]]

c=[ Σy –(b.Σx)] /n

Where:

ȳ= calculated value

c= intercept of the least square line

b= slope of the least square line

To measure the concentration of any substance, we have many methods, one of these methods is spectrophotometry method for measurement of concentration of the sample by applying certain waves either visible light or ultraviolet light.

Visible light range from 400-700 nm, ultraviolet light range from 200-400 nm.

●The Experiment:

The Apparatus:

Spectronic-20 is an instrument which read the absorption for colored solution and used for quantitative analysis to find the concentration of the sample by regression analysis(Linear Regression Line).

The Materials:

*Color Developing Reagent:

   Mercuric chloride           40 gm

   Ferric nitrate                    40 gm

   Hcl (1N)                            120 ml

   D.W.                      ad to  1000 ml

 

*Salicylic acid  

The Procedure:

1- Prepare 250 ml of stock solution of sodium salicylate containing the equivalent of 

200 mg S.A./100 ml.

 

2- From the stock solution of sodium salicylate, use volumetric procedure to accurately prepare solutions containing the equivalent of 50, 40, 30, 20 and 10 mg S.A./100 ml.

 

3- Take 1 ml of each solution and add 5 ml of color developing reagent & mix the solution.

4- Determine the absorbance against a suitable blank at 530 nm.

 

5- Plot absorbance versus concentration of salicylic acid.

6- Apply the least square method to calculate the slope and intercept.

~ Sensitive Balance ~

Sensitive Balance

Is an instrument used in lab as biopharmaceuticto measure the weight of  substances as

(Aspirin (salicylic acid

as shown in pic. below